Annotating Oyster Protein set


ftp://climb.genomics.cn/pub/10.5524/100001_101000/100030/gene_v9/oyster.v9.glean.final.rename.gff.pep.gz

oyster.v9.glean.aa.fasta

modified - original has formatting issues….

oyster_v9_aa_format1.fasta




./blastp -query /Volumes/Bay3/Software/ncbi-blast-2.2.26+/query/oyster_v9_aa_format1.fasta -db /Volumes/Bay3/Software/ncbi-blast-2.2.26+/db/uniprot_sprot -max_target_seqs 1 -evalue 1E-05 -out /Volumes/Bay3/Software/ncbi-blast-2.2.26+/out/oyster_v9_aa_blastp_uniprot_c -num_threads 2 -outfmt 6


oyster_v9_aa_blastp_uniprot_c







robertsmac:bin sr320$ ./blastp -query /Volumes/Bay3/Software/ncbi-blast-2.2.26+/query/oyster_v9_aa_format1.fasta -db /Volumes/Bay3/Software/ncbi-blast-2.2.26+/db/uniprot_sprot -max_target_seqs 1 -evalue 10 -out /Volumes/Bay3/Software/ncbi-blast-2.2.26+/out/oyster_v9_aa_blastp_uniprot_b -num_threads 2 -outfmt 6
Selenocysteine (U) at position 637 replaced by X
Selenocysteine (U) at position 690 replaced by X
Selenocysteine (U) at position 193 replaced by X
Selenocysteine (U) at position 637 replaced by X
Selenocysteine (U) at position 690 replaced by X
Selenocysteine (U) at position 37 replaced by X
Selenocysteine (U) at position 637 replaced by X
Selenocysteine (U) at position 637 replaced by X…………..

oyster_v9_aa_blastp_uniprot_b





refseq_protein

/Volumes/Bay3/CLC_blastdatabases/refseq_protein


./blastp -query /Volumes/Bay3/Software/ncbi-blast-2.2.26+/query/oyster_v9_aa_format1.fasta -db /Volumes/Bay3/CLC_blastdatabases/refseq_protein -max_target_seqs 1 -evalue 1E-05 -out /Volumes/Bay3/Software/ncbi-blast-2.2.26+/out/oyster_v9_aa_blastp_refseq -num_threads 1 -outfmt 6

NEED to retry on node 2